Nettet5. mar. 2024 · Because of these inversions, Lineweaver-Burk plots are commonly referred to as ‘double-reciprocal’ plots. As can be seen at left, the value of K M on a … NettetLineweaver Burk plots are a graphical method of analyzing the Michaelis–Menten equation and the enzyme-substrate-inhibitor relationship. The x-axis in the graph is …
Competitive Inhibition - Chemistry LibreTexts
NettetThis type of inhibition reduces the maximum rate of a chemical reaction without changing the apparent binding affinity of the catalyst for the substrate (K mapp – see Michaelis-Menten kinetics ). When a non-competitive inhibitor is added the Vmax is changed, while the Km remains unchanged. Nettet14. jan. 1999 · (a) Lineweaver-Burk plot showing the effect of uncompetitive inhibition; (b) the same, showing plots for several inhibitor concentrations at fixed enzyme … michigan trails advisory group
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Nettet1. sep. 2024 · As shown in Figure 13.14, when we display kinetic data using as a Lineweaver-Burk plot it is easy to determine which mechanism is in effect. For … Nettet1. sep. 2024 · Another commonly-used plot in examining enzyme kinetics is the Lineweaver-Burk plot, in with to inverse of the feedback rate, \(1/r\), is plotted against the inverse of the substrate concentration \(1 ... Non-competitive inhibition produces plots on the same x-intercept as unobstructed enzyme (\(K_m\) is unaffected) but other slopes ... Nettet10. apr. 2024 · A Lineweaver–Burk plot of substrate p-NPP in the presence of different inhibitor concentrations is shown in Fig. 4A. The experiment revealed that compound 5h intersected in the second quadrant. While Km remained unchanged, Vmax decreased as the inhibitor concentration increased. michigan trailer sales az