Shythdf2
WebFig. 7 LXRalpha and HIVEP2 are functionally essential targets of YTHDF2 in cell proliferation, invasion, cholesterol dysregulation, and tumorigenesis of GBM cells. a Western blotting of … WebJan 8, 2024 · To examine the role of YTHDF2 in GBM tumorigenesis, we used two distinct short hairpin RNAs targeting the 3′UTR (shYTHDF2#1) or the CDS (shYTHDF2#2) of …
Shythdf2
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WebMay 4, 2024 · Total RNA from shYTHDF2 and NTC Hep3B cell subclones was extracted and subjected to RNA sequencing. However, no difference in NANOG, SOX2, OCT4, and KLF4 … WebshRNA Plasmid. shRNA Lentiviral Particles. Description. 10 µM, 50-100 transfections. siRNA products generally consist of pools of three to five target-specific 19-25 nt siRNAs designed to knockdown gene expression. for independent verification of YTHDF2 gene silencing results, individual siRNA duplex components are also available upon request.
WebOct 12, 2024 · Messenger RNA m 6 A modification is shown to regulate local translation in axons. However, how the m 6 A codes in axonal mRNAs are read and decoded by the m 6 … Web(C) RT-qPCR analysis of MYC mRNA expression relative to GAPDH of mRNA from shYTHDF2 TNBC, HR+ and HER2+ breast cancer cell lines. (D) Cumulative distribution of …
WebFeb 10, 2024 · Then shYTHDF2 groups were subsequently overexpressed with MCM2 and MCM5 (shY2+OE-M2/5), with (or without) overexpression of WT/S263A YTHDF2. All the … http://www.jinpanmed.cn/archives/date/2024/04/14
WebAug 5, 2024 · Figure 4. Depletion of YTHDF2 triggers activation of EMT. (A) Cumulative distribution of the fold change in mRNA expression of shYTHDF2 cells over non-targeting …
pentagon shoots down object over alaskaWebYTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a … today\u0027s vision babcock roadWebJan 11, 2024 · The lentiviral system was used to package YTHDF2 or shYTHDF2 lentiviral particles, and then virally infect the gastric cancer cell lines. The finally established … pentagon short cuff pilot glovesWe analyzed The Cancer Genome Atlas (TCGA) and CHOICE datasets of Chinese patients with NSCLC to evaluate the expression profiles of m6A “write”, “eraser”, and “reader” genes (WERs) in LUAD23. The results showed that in both datasets, the mRNA expression levels of METTL3, VIRMA, RBM15, RBM15B, … See more Next, we investigated the effect of YTHDF2 on the aggressive capacities of LUAD in vitro. Our results showed that the ectopic overexpression of YTHDF2 in A549 … See more Given that YTHDF2 promotes mRNA degradation by targeting m6A-modified mRNAs to processing bodies in the cytoplasm, identifying the key factor that … See more pentagon shortsWebFeb 1, 2024 · On one hand, as shown in Fig. S4, neither cell proliferation nor colony-formation was affected when YTHDF2 was either knockdown or knockout by either two … pentagon shopping centreWebMar 23, 2024 · Nature 子刊 客户文章：m6A 修饰参与调控急性缺血卒中恢复的作用机制，采用MeRIP-Seq高通量测序发现，在急性缺血卒中（Acute Ischemic Stroke, AIS）患者的梗死周围皮质组织和动物模型中，mRNA的m6A修饰水平相比对照组显著提高，作者在其中筛选得到了Plpp3（编码促进卒中动物模型血管修复的LPP3蛋白） today\u0027s vision banderaWeb100 of cultured RGC s treated by shYthdf2 (Fig ure 1C,D ; Figure 1 v figure supplement 1G,H ). In contrast, 101 the dendrite branching of RGCs with YTHDF1 KD using shYthdf1 was not significantly different from 102 control shRNA ( Figure 1 v figure supplement 1 I), while YTHDF3 KD using shYthdf3 caused a slight pentagon shortcut