Tsingke tse005 2×t5 super pcr mix colony
WebProduct Details. TransTaq® -T PCR SuperMix is a ready-to-use mixture of TransTaq® -T DNA Polymerase, dNTPs and optimized buffer. The SuperMix is provided at 2× … WebThermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. …
Tsingke tse005 2×t5 super pcr mix colony
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Web2×T5 Super PCR Mix （ Colony） 25. μl 172-dicF . 3: μl TY-DicF-56 . 2ul : TY-DicF-60. 2ul : ddH2O . 18: μl PCR process: 98℃ 5min —— 98℃ 10s —— 60℃ 10s —— 72℃ 10s —— Go … WebUSD $119.00. A hot-start 2X PCR master mix with dye. SapphireAmp Fast PCR mix is well-suited for E.coli- based colony PCR, and colony checks can be completed in about 1 hour. …
Web2.5 TaqMan real-time RT-PCR assay. The novel PCR assay was developed by employing the 2×T5 Fast qPCR Mix (TSE301, TsingKe Biotech, China) on CFX384 Touch Real-Time PCR … WebSep 8, 2015 · PrimeSTAR Max polymerase (2× Master Mix, Takara Bio) was used to generate both altered plasmids. PCR reactions were digested with DpnI for ~2 hours at 37°C and purified with Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). 1 μl of the purified product (125–150 ng DNA) was combined with 25 μl NEB 5-alpha chemically competent …
WebMar 7, 2024 · Pipette 8 µL of LB into PCR tubes and 5 mL (+ antibiotics) into glass culture tubes. Pick a colony off the agar plate and mix it into the LB in a PCR tube. Transfer 5 µL …
Web2×T5 Super PCR Mix (Colony) TSE005 产品说明： 目录号： T5 DNA 聚合酶是新型DNA 聚合酶，由经过改造的Taq DNA 聚合酶融合Sso7d DNA结合域而得来，具有极高的扩增 速度 …
Web2×T5 Super PCR Mix（Colony）. ¥ 395.00. 货号：TSE005. 规格：. 5×1 mL. 前往科邦邦商城下单. 产品简介. 合成基因组产品及服务. 引物合成. how to secure loose railingWebNov 1, 2024 · The reaction mixture was incubated for 60 min at 42°C and stored at −20°C until required. Single RT‐PCR. Single RT‐PCR was performed in a 25 µl reaction mixture containing 1·5 µl of cDNA, 22·5 µl of 1·1× T3 Super PCR Mix (Tsingke, Beijing, China) and 0·5 µl of forward and reverse primer each (20 µmol l −1 stock). how to secure lumber in truck bedWeb2 O 12.5 µL 1 µL 1 µL 见标注 Up to 25 µL 25 µL 2 µL 2 µL 见标注 Up to 50 µL 1× 0.4 µM 0.4 µM · 请使用高质量模板。 · 请勿使用dUTP和含有尿嘧啶的引物与模板。 · PCR Mix应避免反复冻融，短期内多次使用可置于2~8℃保存。使用前， Mix解冻后应充分混匀。 注意事项 .推 … how to secure loose sidingWeb2 ×T5 Super PCR Mix (Colony) 5. Initial denaturation. 98. 30s. 1 × . Forward primer . 0.2. Denaturation. 98. 10s. 29 × . Feverse primer . 0.2. Annealing. T m-5. 30s. Template. 2. … how to securely dispose of old cell phonesWeb我们联系：[email protected]。 2×T5 Super PCR Mix (PAGE) TSE006 产品组成： PCR体系与条件： 目录号： 2×T5 Super PCR Mix (PAGE) 5×1.0 ml Component 2×T5 Super PCR Mix (PAGE) 10 μM Forward Primer 10 μM Reverse Primer Template DNA Nuclease-Free Water Final Concentration 1× 0.4 μM 0.4 μM <1 μg 推荐反应体系 ... how to securely create a network folder on piWebJun 9, 2024 · Tsingke Biotech leads the way in the development & manufacturing of genomic and biosynthesis products for global researchers. ... 2×T5 Supper PCR … how to securely access the dark webWeb4 Use 1 µl of reaction for colony PCR (T5 Taq Polymerase) 5 ... Concentration 2XT5 Super PCR Mix 5ul 1x 10uM Forward Primer 0.4ul 0.4uM 10uM Reverse Primer 0.4ul 0.4uM Template DNA 1ul <1ug how to securely dispose of a computer